Application of a monoclonal antibody to estimate rabbit fibrinopeptide A released by habutobin.
Toxicon
; 32(11): 1413-23, 1994 Nov.
Article
en En
| MEDLINE
| ID: mdl-7886699
We reported previously that habutobin, one of the type A thrombin-like enzymes, releases fibrinopeptide A alone from rabbit fibrinogen. To evaluate the effective action of habutobin in experiments using rabbit for the treatment of thrombosis, we attempted to develop an immunological method for measuring the fibrinopeptide A level in the circulating blood of rabbit. The purified rabbit fibrinopeptide A was coupled to keyhole limpet hemocyanin and BALB/c mice were immunized with the resultant fibrinopeptide A-hemocyanin conjugate. The spleen cells of an immunized mouse were fused with myeloma cells (P3-X63-Ag8-U1). As a result, one hybridoma (a-F-7) was selected, which secreted an antibody against rabbit fibrinopeptide A. Using this monoclonal antibody, we developed a competitive enzyme-linked immunoassay for estimating rabbit fibrinopeptide A. It was able to measure rabbit fibrinopeptide A contained in bentonite defibrinated plasma. This competitive enzyme-linked immunoassay should be useful for determining the fibrinopeptide A level in the circulating blood of rabbits, using plasma defibrinated by bentonite.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fibrinopéptido A
/
Serina Endopeptidasas
/
Venenos de Crotálidos
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Toxicon
Año:
1994
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido