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[Cytogenetics and molecular studies confirm the histopathologic diagnosis of chronic myeloproliferative diseases]. / Zytogenetik und molekulare Untersuchungen sichern die histopathologischen Diagnosen von chronischen myeloproliferativen Erkrankungen ab.
Werner, M; Nolte, M; Ewing, M; Kaloutsi, V; Kausche, F; Georgii, A.
Afiliación
  • Werner M; Pathologisches Institut, Medizinische Hochschule Hannover.
Pathologe ; 16(1): 41-5, 1995 Jan.
Article en De | MEDLINE | ID: mdl-7886013
The histopathological classification of chronic myeloproliferative disorders can be supported by applying cytogenetics and molecular genetics to the analysis of bone marrow or blood cells, as demonstrated in 253 cases evaluated. The Philadelphia translocation (9;22) is the most important genetic parameter, being specific for chronic myeloid leukemia. Conventional methods for the detection of the t(9;22) are karyotyping and Southern blot analysis of the bcr gene. The newly established technique of fluorescence in situ hybridization (FISH) allows visualization of bcr-abl fusion even in non dividing cells. Molecular cytogenetics for t(9;22) yield results that are rapid and reliable as well as easily quantifiable.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Tirosina Quinasas / Aberraciones Cromosómicas / Trastornos Mieloproliferativos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: De Revista: Pathologe Año: 1995 Tipo del documento: Article Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Tirosina Quinasas / Aberraciones Cromosómicas / Trastornos Mieloproliferativos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: De Revista: Pathologe Año: 1995 Tipo del documento: Article Pais de publicación: Alemania