G1 control in mammalian cells.
J Cell Sci Suppl
; 18: 69-73, 1994.
Article
en En
| MEDLINE
| ID: mdl-7883795
Cyclin-dependent kinases (Cdks) control the major cell cycle transitions in eukaryotic cells. On the basis of a variety of experiments where cyclin function either is impaired or enhanced, D-type cyclins as well as cyclins E and A have been linked to G1 and G1/S phase roles in mammalian cells. We therefore sought to determine if agents that block the G1/S phase transition do so at the level of regulating the Cdk activities associated with these cyclins. A variety of conditions that lead to G1 arrest were found to correlate with accumulation of G1-specific Cdk inhibitors, including treatment of fibroblasts with ionizing radiation, treatment of epithelial cells with TGF-beta, treatment of HeLa cells with the drug lovastatin, and removal of essential growth factors from a variety of different cell types. Mechanistically, inhibition of Cdks was found to involve the stoichiometric binding of Cdk inhibitor proteins. p21Waf1/Cip1 was associated with DNA damage induced arrest while p27Kip1/p28Ick1 accumulated under a variety of antiproliferative conditions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fase G1
/
Ciclinas
/
Quinasas Ciclina-Dependientes
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Sci Suppl
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Reino Unido