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Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes.
Batista, J A; Teixeira, S M; Donelson, J E; Kirchhoff, L V; de Sá, C M.
Afiliación
  • Batista JA; Departamento de Biologia Celular, Universidade de Brasilia, Brazil.
Mol Biochem Parasitol ; 67(2): 301-12, 1994 Oct.
Article en En | MEDLINE | ID: mdl-7870134
We have characterized the biochemical properties of a 66-kDa poly(A)-binding protein (PABP1) in the protozoan Trypanosoma cruzi and isolated two classes of cDNAs encoding the protein. In concordance, Southern blots showed the presence of 2 gene copies. The two cDNA classes differ in the length of adenosine-rich segments in the 5' untranslated region and in point changes scattered throughout the sequence, but their 1650-bp open reading frames encode identical proteins. A single mRNA of 5.5 kb was detected, indicating that the noncoding regions are unusually long. Both the mRNA and the protein are constitutively expressed in all stages of T. cruzi life cycle. The biochemical properties and sequence comparisons show that the T. cruzi PABP1 is similar to the PABP1 of other eukaryotic organisms. These results indicate that PABP1 has been conserved throughout eukaryotic evolution.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / Proteínas de Unión al ARN / Genes Protozoarios Límite: Animals Idioma: En Revista: Mol Biochem Parasitol Año: 1994 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / Proteínas de Unión al ARN / Genes Protozoarios Límite: Animals Idioma: En Revista: Mol Biochem Parasitol Año: 1994 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos