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NMR lipid profiles of cells, tissues, and body fluids: proton NMR analysis of human erythrocyte lipids.
Adosraku, R K; Choi, G T; Constantinou-Kokotos, V; Anderson, M M; Gibbons, W A.
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  • Adosraku RK; University Industry Centre for Pharmaceutical Research, School of Pharmacy, University of London.
J Lipid Res ; 35(11): 1925-31, 1994 Nov.
Article en En | MEDLINE | ID: mdl-7868971
One- and two-dimensional high resolution NMR spectroscopy was applied to determine quantitatively and qualitatively the lipids extracted from human erythrocyte membranes. The relative amounts of the major lipids were determined from the spectra of unfractionated lipid extracts. After HPLC fractionation of the lipid extracts and NMR analysis of the fractions, it was possible to determine the features of the component lipids of each lipid class and to compare, especially, the fatty acid types and composition of the individual major glycerophospholipids. The results of this proton NMR analysis were compared to those obtained elsewhere using classical lipid analytical techniques and found to be in substantial agreement.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectroscopía de Resonancia Magnética / Membrana Eritrocítica / Lípidos Límite: Humans Idioma: En Revista: J Lipid Res Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectroscopía de Resonancia Magnética / Membrana Eritrocítica / Lípidos Límite: Humans Idioma: En Revista: J Lipid Res Año: 1994 Tipo del documento: Article Pais de publicación: Estados Unidos