Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas.
Rev Inst Med Trop Sao Paulo
; 36(3): 205-10, 1994.
Article
en En
| MEDLINE
| ID: mdl-7855483
Hydatid disease in tropical areas poses a serious diagnostic problem due to the high frequence of cross-reactivity with other endemic helminthic infections. The enzyme-linked-immunosorbent assay (ELISA) and the double diffusion arc 5 showed respectively a sensitivity of 73% and 57% and a specificity of 84-95% and 100%. However, the specificity of ELISA was greatly increased by using ovine serum and phosphorylcholine in the diluent buffer. The hydatic antigen obtained from ovine cyst fluid showed three main protein bands of 64, 58 and 30 KDa using SDS PAGE and immunoblotting. Sera from patients with onchocerciasis, cysticercosis, toxocariasis and Strongyloides infection cross-reacted with the 64 and 58 KDa bands by immunoblotting. However, none of the analyzed sera recognized the 30 KDa band, that seems to be specific in this assay. The immunoblotting showed a sensitivity of 80% and a specificity of 100% when used to recognize the 30 KDa band.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clima Tropical
/
Ensayo de Inmunoadsorción Enzimática
/
Immunoblotting
/
Equinococosis
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Año:
1994
Tipo del documento:
Article
País de afiliación:
Venezuela
Pais de publicación:
Brasil