Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum.

Robert, L S; Gerster, J; Allard, S; Cass, L; Simmonds, J

Robert, L S; Gerster, J; Allard, S; Cass, L; Simmonds, J.

Afiliación

Robert LS; Plant Research Centre, Central Experimental Farm, Ottawa, Ont., Canada.

Plant J ; 6(6): 927-33, 1994 Dec.

Article en En | MEDLINE | ID: mdl-7849761

RESUMEN

Two highly homologous Brassica napus flower cDNA clones, Sta 41-2 and Sta 41-9, were isolated and characterized. These clones were shown to correspond to genes expressed in the tapetum from the early uninucleate microspore stage to the dinucleate stage. The predicted Sta 41-2 and Sta 41-9 proteins possessed characteristics similar to oleosins such as a polar N-terminal domain, a large relatively conserved hydrophobic domain and a long C-terminal domain which consisted of four different groups of repeats. In addition, like oleosins, the Sta 41-2 and Sta 41-9 proteins have a basic pI, lack a signal peptide and are found in a tissue which accumulates lipids in small lipid bodies.

Asunto(s)

Brassica/genética; Expresión Génica; Genes de Plantas; Proteínas de Plantas/genética; Secuencia de Aminoácidos; Secuencia de Bases; Brassica/metabolismo; Clonación Molecular; Datos de Secuencia Molecular; Proteínas de Plantas/biosíntesis; Proteínas de Plantas/química; Conformación Proteica; Secuencias Repetitivas de Ácidos Nucleicos; Homología de Secuencia de Aminoácido; Homología de Secuencia de Ácido Nucleico

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Brassica / Expresión Génica / Genes de Plantas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 1994 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido

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