Improved method for preparing N-hydroxysuccinimide ester-containing polymers for affinity chromatography.
Bioconjug Chem
; 5(5): 491-2, 1994.
Article
en En
| MEDLINE
| ID: mdl-7849082
N,N,N',N'-Tetramethyl(succinimido) uronium tetrafluoroborate is proposed as a reagent of choice for the activation of carboxyl groups and formation of N-hydroxysuccinimide esters on polymers. Unlike conventional methods which generate unstable gels, the reaction is appropriate for hydroxy-containing resins like Sepharose, cellulose, and dextran. The yields of activation and subsequent coupling capacity for ligands and proteins are very high. The respective columns can be used for affinity chromatography and immobilization of proteins.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Succinimidas
/
Cromatografía de Afinidad
/
Compuestos de Metilurea
Idioma:
En
Revista:
Bioconjug Chem
Asunto de la revista:
BIOQUIMICA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Israel
Pais de publicación:
Estados Unidos