Continuous beds. Their applicability for immobilization of proteins.
Biomed Chromatogr
; 8(4): 165-9, 1994.
Article
en En
| MEDLINE
| ID: mdl-7812119
An epoxy-activated continuous bed can be prepared for immobilization of proteins in a simple, rapid, and cost-effective way. The concentration of epoxy groups on the continuous bed was as high as 600 mumol/mL compressed bed (compression of the bed decreases the peak broadening). Human transferrin, human serum albumin and particularly urease were employed as model proteins. The immobilization of urease was virtually completed within 1 h in 1 M potassium phosphate, pH 7.4. The binding capacity was 97 mg of urease/mL compressed bed. This bed is of clinical interest, since it is inexpensive to prepare and permits reproducible enzymatic determination of urea in serum and urine (the chromatographic step is finished within 1-2 min).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ureasa
/
Albúmina Sérica
/
Transferrina
/
Cromatografía Líquida de Alta Presión
/
Resinas Epoxi
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biomed Chromatogr
Año:
1994
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido