Copper, zinc superoxide dismutase in Escherichia coli: periplasmic localization.
Arch Biochem Biophys
; 319(2): 508-11, 1995 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-7786035
Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD. Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Superóxido Dismutasa
/
Escherichia coli
Límite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos