Detailed deletion mapping of chromosome 6q in borderline epithelial ovarian tumors.

Rodabaugh, K J; Blanchard, G; Welch, W R; Bell, D A; Berkowitz, R S; Mok, S C

Rodabaugh, K J; Blanchard, G; Welch, W R; Bell, D A; Berkowitz, R S; Mok, S C.

Afiliación

Rodabaugh KJ; Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.

Cancer Res ; 55(10): 2169-72, 1995 May 15.

Article en En | MEDLINE | ID: mdl-7743519

RESUMEN

We have used PCR amplification of tandem repeats and Southern blot analysis to study the pattern of allelic loss at chromosome 6q in borderline ovarian tumors and compared that with invasive ovarian carcinomas. DNA from 46 borderline ovarian tissues, 20 invasive ovarian tumor tissues, together with corresponding uninvolved (control) tissues was used. The invasive tumors demonstrated the highest percentage of loss of heterozygosity (13 of 45 informative cases, 29%) at the 6q25-27 locus site. In contrast, the borderline ovarian tumors showed only an 11% frequency of loss of heterozygosity (3 of 26). Our results display a sharp contrast in the pattern of loss of heterozygosity between invasive and borderline ovarian tumors and suggest that allelic loss at chromosome 6q may not be involved in the development of borderline ovarian tumors.

Asunto(s)

Alelos; Cromosomas Humanos Par 6/genética; Eliminación de Gen; Neoplasias Ováricas/genética; Southern Blotting; Femenino; Humanos; Neoplasias Ováricas/patología; Reacción en Cadena de la Polimerasa

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Cromosomas Humanos Par 6 / Eliminación de Gen / Alelos Límite: Female / Humans Idioma: En Revista: Cancer Res Año: 1995 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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