Anti-retinoic acid monoclonal antibody localizes all-trans-retinoic acid in target cells and blocks normal development in early quail embryo.
Dev Biol
; 168(2): 225-34, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7729565
Avian cardiovascular development is vitamin A-dependent, and retinoic acid has been suggested to be active in this important developmental event. We report here that a monoclonal antibody against all-trans-retinoic acid blocks normal embryonic development in the quail causing cardiovascular abnormalities typical of avian vitamin A deficiency. In whole-mount preparations of stage 5 normal quail embryos the fluorescence associated with the antiretinoic acid monoclonal antibody localizes in Hensen's node and in caudal area. In stage 7-8 embryos fluorescence localizes in heart-forming areas as well as in head mesenchyme, in Hensen's node, in nephrotome, and in caudal area. These studies are the first to localize endogenous all-trans-retinoic acid during very early stages of normal avian development. We propose that all-trans-retinoic acid is biosynthesized in its target cells during early avian embryo-genesis and that the availability of this signal molecule is spatiotemporally regulated. We conclude that all-trans-retinoic acid or a closely related metabolite is the physiological form of vitamin A required for normal cardiovascular development and for other very early developmental events in the quail embryo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Codorniz
/
Tretinoina
Límite:
Animals
Idioma:
En
Revista:
Dev Biol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos