Retinoic acid enhances connexin43 expression at the post-transcriptional level in rat liver epithelial cells.
Cell Biochem Funct
; 13(1): 69-77, 1995 Mar.
Article
en En
| MEDLINE
| ID: mdl-7720192
The mechanism by which all-trans retinoic acid (RA) stimulates gap junctional intercellular communication (GJIC) in the rat liver epithelial cell line. IAR203, was investigated. When RA, at 0.1 microM for 24-48 h, enhanced the dye transfer in IAR203 cells (x 1.4), it increased the amount of connexin43 (Cx43) in the cell-cell contact regions of the plasma membrane, as evidenced by analysis by Western blot and by immunofluorescence. It had no effect on the level of Cx43 mRNA. Freeze-fracture analysis of the size of gap junctions revealed an increase of the proportion of small gap junctions in RA-treated cells. We conclude that, in IAR203 cells, RA stimulates GJIC by acting at the post-transcriptional level of Cx43 regulation. The possibility that RA acts indirectly on the regulation of Cx43 expression, and increases the half-life of Cx43 by inducing adhesion molecules is discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tretinoina
/
Procesamiento Proteico-Postraduccional
/
Conexina 43
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
Cell Biochem Funct
Año:
1995
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido