Altered glycosylation of pituitary gonadotropins in anorexia nervosa: an alternative explanation for amenorrhea.
Eur J Endocrinol
; 132(4): 450-5, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7711883
To investigate the relevance of glycoprotein polymorphism to gonadotropin bioactivity in vivo, plasma follicle-stimulating hormone (FSH) and luteinizing hormone (LH), 17 beta-estradiol (E2), testosterone and sex hormone binding globulin (SHBG) levels in 17 amenorrheic women affected with anorexia nervosa (14-29 years) and 10 age-matched normally cycling women were evaluated. Plasma FSH and LH levels were assayed using radioimmunoassay (RIA) and immunoradiometric assay (IRMA) methods, before and after concanavalin A-Sepharose (Con A) affinity chromatography. Significant RIA-IRMA differences in FSH and LH plasma values were present only in women with anorexia nervosa (p < 0.005). Moreover, in these patients both FSH and LH showed a reduced binding to the Con A, expressed as a percentage of unbound, suggesting altered glycosylation of these moieties. In conclusion, these findings hypothesize the involvement of glycosylation polymorphism in RIA-IRMA differences; support the usefulness of both RIA and IRMA methods in FSH and LH evaluation, before and after Con A chromatography; and suggest a new pathogenetic pathway to explain amenorrhea in anorexia nervosa.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Anorexia Nerviosa
/
Amenorrea
/
Gonadotropinas
Límite:
Adolescent
/
Adult
/
Female
/
Humans
Idioma:
En
Revista:
Eur J Endocrinol
Asunto de la revista:
ENDOCRINOLOGIA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido