Your browser doesn't support javascript.
loading
Altered glycosylation of pituitary gonadotropins in anorexia nervosa: an alternative explanation for amenorrhea.
Tommaselli, A P; Valentino, R; Savastano, S; Randazzo, G; Scalfi, L; Contaldo, F; Dorato, M; Lombardi, G.
Afiliación
  • Tommaselli AP; Chair of Endocrinology, CEOS, National Research Council (CNR), Naples, Italy.
Eur J Endocrinol ; 132(4): 450-5, 1995 Apr.
Article en En | MEDLINE | ID: mdl-7711883
To investigate the relevance of glycoprotein polymorphism to gonadotropin bioactivity in vivo, plasma follicle-stimulating hormone (FSH) and luteinizing hormone (LH), 17 beta-estradiol (E2), testosterone and sex hormone binding globulin (SHBG) levels in 17 amenorrheic women affected with anorexia nervosa (14-29 years) and 10 age-matched normally cycling women were evaluated. Plasma FSH and LH levels were assayed using radioimmunoassay (RIA) and immunoradiometric assay (IRMA) methods, before and after concanavalin A-Sepharose (Con A) affinity chromatography. Significant RIA-IRMA differences in FSH and LH plasma values were present only in women with anorexia nervosa (p < 0.005). Moreover, in these patients both FSH and LH showed a reduced binding to the Con A, expressed as a percentage of unbound, suggesting altered glycosylation of these moieties. In conclusion, these findings hypothesize the involvement of glycosylation polymorphism in RIA-IRMA differences; support the usefulness of both RIA and IRMA methods in FSH and LH evaluation, before and after Con A chromatography; and suggest a new pathogenetic pathway to explain amenorrhea in anorexia nervosa.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anorexia Nerviosa / Amenorrea / Gonadotropinas Límite: Adolescent / Adult / Female / Humans Idioma: En Revista: Eur J Endocrinol Asunto de la revista: ENDOCRINOLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anorexia Nerviosa / Amenorrea / Gonadotropinas Límite: Adolescent / Adult / Female / Humans Idioma: En Revista: Eur J Endocrinol Asunto de la revista: ENDOCRINOLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido