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Observation of the helical structure of the bacterial polysaccharide acetan by atomic force microscopy.
Kirby, A R; Gunning, A P; Morris, V J; Ridout, M J.
Afiliación
  • Kirby AR; Institute of Food Research, Colney, Norwich, United Kingdom.
Biophys J ; 68(1): 360-3, 1995 Jan.
Article en En | MEDLINE | ID: mdl-7711262
A method has been developed that has been found to give reproducible images of uncoated polysaccharides by Atomic Force Microscopy (AFM). Aqueous solutions of the polysaccharide are deposited as drops onto freshly cleaved mica surfaces, air dried, and then imaged under butanol. The method has been used to obtain images of the bacterial polysaccharide acetan. In regions within the deposited sample, where the molecules are aligned side-by-side, it has been possible to observe a periodic structure along the polysaccharide chain, attributable to the helical structure of acetan.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Microscopía de Fuerza Atómica Idioma: En Revista: Biophys J Año: 1995 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Microscopía de Fuerza Atómica Idioma: En Revista: Biophys J Año: 1995 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos