Presence of a novel form of phosphatidylinositol 4-kinase in rat liver.
FEBS Lett
; 361(2-3): 282-6, 1995 Mar 20.
Article
en En
| MEDLINE
| ID: mdl-7698339
Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microsomas Hepáticos
/
Fosfotransferasas (Aceptor de Grupo Alcohol)
/
Isoenzimas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1995
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido