Some new properties of DNA-YOYO-3 homodimer complexes revealed by electrophoresis and fluorescence lifetime measurements.
Biochem Mol Biol Int
; 34(6): 1189-96, 1994 Dec.
Article
en En
| MEDLINE
| ID: mdl-7696991
The DNA bis-intercalator oxazole homodimer (YOYO-3: 1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methy l- 2,3-dihydro-(benzo-1,3-oxazole)-2-methylidene]-quinolinium tetraiodide) specifically alters the electrophoretic pattern of covalently closed circular (CCC) DNA molecules. Thus, YOYO-3 seems to remove the CCC DNA supercoils and induces the appearance of additional bands by changing the linking number. It also promotes an unusual "star" activity for the restriction enzyme Hind III. Fluorescence lifetime measurements indicate that YOYO-3 is capable of binding to DNA by bis- and mono-intercalation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Quinolinio
/
Benzoxazoles
/
ADN Circular
/
Sustancias Intercalantes
/
Conformación de Ácido Nucleico
Idioma:
En
Revista:
Biochem Mol Biol Int
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Rumanía
Pais de publicación:
Reino Unido