Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors.
Cell
; 74(5): 823-32, 1993 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-7690686
In the absence of growth factors, hemopoietic cells die rapidly by the process of apoptosis. Transfection of the human bcl-2 gene into an interleukin-3 (IL-3)-dependent, multipotent hemopoietic cell line allowed these cells to survive in the absence of IL-3, both in serum-containing and serum-deprived conditions, and this survival was accompanied by multilineage differentiation. Moreover, single cell experiments showed that differentiation could occur in the absence of cell division. While these data do not rule out the possibility that growth factors can influence the lineage choice of multipotent cells, they suggest that exposure to growth factors may not be obligatory for the differentiation of stem cells. The data also support the hypothesis that differentiation is intrinsically determined and that the role of the hemopoietic growth factors is enabling rather than inductive.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proto-Oncogenes
/
Proteínas Proto-Oncogénicas
/
Apoptosis
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell
Año:
1993
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos