Promoter opening (melting) and transcription initiation by RNA polymerase I requires neither nucleotide beta,gamma hydrolysis nor protein phosphorylation.
Nucleic Acids Res
; 21(14): 3233-8, 1993 Jul 11.
Article
en En
| MEDLINE
| ID: mdl-7688114
With some bacterial RNA polymerases and in eukaryotic RNA polymerase II, DNA melting during initiation requires the coupling of energy derived from beta,gamma hydrolysis of ATP. A detailed analysis of this possible requirement for eukaryotic RNA polymerase I reveals no such requirement. However, in some cases, beta,gamma non-hydrolyzable derivatives (beta,gamma imido or methylene) of nucleotide substrates have been found to significantly inhibit transcription initiation because of their inefficient use as the first nucleotide of the transcript. In addition, the results presented here show that protein kinase activity is not required as an integral part of transcription initiation by RNA polymerase I. Prior phosphorylation of proteins participating in the process is not ruled out.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
ARN Polimerasa I
/
Regiones Promotoras Genéticas
/
Nucleótidos
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Reino Unido