1H, 13C, and 15N assignments and secondary structure of the FK506 binding protein when bound to ascomycin.

Xu, R X; Nettesheim, D; Olejniczak, E T; Meadows, R; Gemmecker, G; Fesik, S W

Xu, R X; Nettesheim, D; Olejniczak, E T; Meadows, R; Gemmecker, G; Fesik, S W.

Afiliación

Xu RX; Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Illinois 60064.

Biopolymers ; 33(4): 535-50, 1993 Apr.

Article en En | MEDLINE | ID: mdl-7682113

RESUMEN

The 1H, 13C, and 15N resonances of FKBP when bound to the immunosuppressant, ascomycin, were assigned using a computer-aided analysis of heteronuclear double and triple resonance three-dimensional nmr spectra of [U-15N]FKBP/ascomycin and [U-15N,13C]FKBP/ascomycin. In addition, from a preliminary analysis of two heteronuclear four-dimensional data sets, 3JHN,H alpha coupling constants, amide exchange data, and the differences between the C alpha and C beta chemical shifts of FKBP to random coil values, the secondary structure of FKBP when bound to ascomycin was determined. The secondary structure of FKBP when bound to ascomycin in solution closely resembled the x-ray structure of the FKBP/FK506 complex but differed in some aspects from the structure of uncomplexed FKBP in solution.

Asunto(s)

Proteínas Portadoras/química; Tacrolimus/metabolismo; Secuencia de Aminoácidos; Proteínas Portadoras/metabolismo; Humanos; Técnicas In Vitro; Espectroscopía de Resonancia Magnética; Datos de Secuencia Molecular; Estructura Secundaria de Proteína; Proteínas Recombinantes/química; Proteínas Recombinantes/metabolismo; Tacrolimus/análogos & derivados; Proteínas de Unión a Tacrolimus

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Portadoras / Tacrolimus Límite: Humans Idioma: En Revista: Biopolymers Año: 1993 Tipo del documento: Article Pais de publicación: Estados Unidos

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