Toxicity of dopamine to mouse neuroblastoma x rat glioma hybrid (NG108) cells in vitro.
Zhongguo Yao Li Xue Bao
; 16(4): 294-6, 1995 Jul.
Article
en En
| MEDLINE
| ID: mdl-7668093
AIM: To study toxicity of dopamine to mouse neuroblastoma x rat glioma hybrid (NG108) cells. METHODS: Cell viability was estimated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Dopamine at 100 mumol L-1 was toxic when added to cultures 24 h after plating. The cell viability was about 1/4 of control. Toxicity did not seem to be mediated by dopaminergic receptors because the dopaminergic antagonists sulpiride and Sch-23390 did not block the toxic effect of dopamine. Catalase 50 kU L-1, superoxide dismutase 50 kU L-1 and l-ascorbic acid 200 mumol L-1 blocked the dopamine (125 mumol L-1) toxicity and elevated the cell viability from 25.9 +/- 11.0% to 74.8 +/- 4.4%, 72.3 +/- 4.5% and 71.4 +/- 2.3%, respectively. CONCLUSION: Dopamine toxicity to NG108 cells was mainly attributed to the oxidation of dopamine and its toxic by-products, eg, H2O2.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dopamina
/
Glioma
/
Neuroblastoma
Límite:
Animals
Idioma:
En
Revista:
Zhongguo Yao Li Xue Bao
Año:
1995
Tipo del documento:
Article
Pais de publicación:
China