The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb.

Craven, R J; Cance, W G; Liu, E T

Craven, R J; Cance, W G; Liu, E T.

Afiliación

Craven RJ; Lineberger Comprehensive Cancer Center, Department of Surgery, University of North Carolina at Chapel Hill 27599, USA.

Cancer Res ; 55(18): 3969-72, 1995 Sep 15.

Article en En | MEDLINE | ID: mdl-7664264

RESUMEN

Rak is a nuclear tyrosine kinase containing Src homology 2 and 3 domains at its NH2 terminus. We report here that the retinoblastoma tumor susceptibility gene product pRb associates with Rak in vivo and in vitro. Rak binds in the A/B pocket region of pRb, a region that is frequently mutated in human cancer, during the G1 and S phases of the cell cycle. Furthermore, Rak expression is elevated in G1, and transfection of Rak into NIH 3T3 cells results in a significant decrease in the number of emerging colonies. Thus, Rak is a tyrosine kinase with growth suppressing activity that may function, in part, through its interaction with pRb.

Asunto(s)

Proteínas de Neoplasias; Proteínas Tirosina Quinasas/metabolismo; Proteína de Retinoblastoma/metabolismo; Células 3T3; Secuencia de Aminoácidos; Animales; Humanos; Ratones; Datos de Secuencia Molecular; Fosforilación; Células Tumorales Cultivadas; Familia-src Quinasas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Tirosina Quinasas / Proteína de Retinoblastoma / Proteínas de Neoplasias Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Cancer Res Año: 1995 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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