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Putative neurohemal areas in the peripheral nervous system of an insect, Gryllus bimaculatus, revealed by immunocytochemistry.
Helle, J; Dircksen, H; Eckert, M; Nässel, D R; Spörhase-Eichmann, U; Schürmann, F W.
Afiliación
  • Helle J; I. Zoologisches Institut, Abteilung für Zellbiologie, Universität Göttingen, Germany.
Cell Tissue Res ; 281(1): 43-61, 1995 Jul.
Article en En | MEDLINE | ID: mdl-7621526
The morphology and position of putative neurohemal areas in the peripheral nervous system (ventral nerve cord and retrocerebral complex) of the cricket Gryllus bimaculatus are described. By using antisera to the amines dopamine, histamine, octopamine, and serotonin, and the neuropeptides crustacean cardioactive peptide, FMRFamide, leucokinin 1, and proctolin, an extensive system of varicose fibers has been detected throughout the nerves of all neuromeres, except for nerve 2 of the prothoracic ganglion. Immunoreactive varicose fibers occur mainly in a superficial position at the neurilemma, indicating neurosecretory storage and release of neuroactive compounds. The varicose fibers are projections from central or peripheral neurons that may extend over more than one segment. The peripheral fiber varicosities show segment-specific arrangements for each of the substances investigated. Immunoreactivity to histamine and octopamine is mainly found in the nerves of abdominal segments, whereas serotonin immunoreactivity is concentrated in subesophageal and terminal ganglion nerves. Immunoreactivity to FMRFamide and crustacean cardioactive peptide is widespread throughout all segments. Structures immunoreactive to leucokinin 1 are present in abdominal nerves, and proctolin immunostaining is found in the terminal ganglion and thoracic nerves. Codistribution of peripheral varicose fiber plexuses is regularly seen for amines and peptides, whereas the colocalization of substances in neurons has not been detected for any of the neuroactive compounds investigated. The varicose fiber system is regarded as complementary to the classical neurohemal organs.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insectos / Sistema Nervioso Límite: Animals Idioma: En Revista: Cell Tissue Res Año: 1995 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insectos / Sistema Nervioso Límite: Animals Idioma: En Revista: Cell Tissue Res Año: 1995 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania