Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs.
Int J Parasitol
; 25(3): 359-65, 1995 Mar.
Article
en En
| MEDLINE
| ID: mdl-7601594
In the current study, molecular techniques were evaluated for the species identification of individual strongyle eggs. Adult worms of Strongylus edentatus, S. equinus and S. vulgaris were collected at necropsy from horses from Australia and the U.S.A. Genomic DNA was isolated and a ribosomal transcribed spacer (ITS-2) amplified and sequenced using polymerase chain reaction (PCR) techniques. The length of the ITS-2 sequence of S. edentatus, S. equinus and S. vulgaris ranged between 217 and 235 nucleotides. Extensive sequence analysis demonstrated a low degree (0-0.9%) of intraspecific variation in the ITS-2 for the Strongylus species examined, whereas the levels of interspecific differences (13-29%) were significantly greater. Interspecific differences in the ITS-2 sequences allowed unequivocal species identification of single worms and eggs using PCR-linked restriction fragment length polymorphism. These results demonstrate the potential of the ribosomal spacers as genetic markers for species identification of single strongyle eggs from horse faeces.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Parasitología
/
Infecciones Equinas por Strongyloidea
/
Strongylus
/
ADN Ribosómico
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Int J Parasitol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Australia
Pais de publicación:
Reino Unido