Neuroglycan C, a novel membrane-spanning chondroitin sulfate proteoglycan that is restricted to the brain.

Watanabe, E; Maeda, N; Matsui, F; Kushima, Y; Noda, M; Oohira, A

Watanabe, E; Maeda, N; Matsui, F; Kushima, Y; Noda, M; Oohira, A.

Afiliación

Watanabe E; Department of Perinatology and Neuroglycoscience, Institute for Developmental Research, Aichi, Japan.

J Biol Chem ; 270(45): 26876-82, 1995 Nov 10.

Article en En | MEDLINE | ID: mdl-7592931

RESUMEN

Monoclonal antibodies were raised to membrane-bound proteoglycans derived from rat brain, and four monoclonal antibodies that recognized a 150-kDa chondroitin sulfate proteoglycan with a core glycoprotein of 120 kDa were obtained. Immunohistological study revealed that the proteoglycan was associated with developing neurons. We screened rat brain cDNA libraries using the four monoclonal antibodies and isolated overlapping cDNA clones that encoded the entire core protein of 514 amino acids plus a 30-residue signal peptide. The deduced amino acid sequence suggested an integral membrane protein divided into five structurally different domains: an N-terminal domain to which chondroitin sulfate chains might be attached, a basic amino acid cluster consisting of seven arginine and two lysine residues, a cysteine-containing domain, a membrane-spanning segment, and a C-terminal cytoplasmic domain of 95 amino acids. On Northern blots, the cDNA hybridized with a single mRNA of 3.1 kilobases that was detectable in brains of neonatal and adult rats but not in kidney, liver, lung, and muscle of either. The sequence of the proteoglycan did not exhibit significant homology to any other known protein, indicating that the proteoglycan, designated neuroglycan C, is a novel integral membrane proteoglycan.

Asunto(s)

Encéfalo/metabolismo; Proteoglicanos Tipo Condroitín Sulfato/metabolismo; Proteínas de la Matriz Extracelular; Proteínas de la Membrana/metabolismo; Proteínas del Tejido Nervioso/metabolismo; Agrecanos; Secuencia de Aminoácidos; Animales; Anticuerpos Monoclonales; Secuencia de Bases; Proteoglicanos Tipo Condroitín Sulfato/genética; Proteoglicanos Tipo Condroitín Sulfato/inmunología; Clonación Molecular; Cartilla de ADN/genética; ADN Complementario/genética; Lectinas Tipo C; Proteínas de la Membrana/genética; Proteínas de la Membrana/inmunología; Datos de Secuencia Molecular; Estructura Molecular; Proteínas del Tejido Nervioso/genética; Proteínas del Tejido Nervioso/inmunología; Proteoglicanos/genética; ARN Mensajero/genética; ARN Mensajero/metabolismo; Ratas; Ratas Sprague-Dawley; Distribución Tisular

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoglicanos Tipo Condroitín Sulfato / Encéfalo / Proteínas de la Matriz Extracelular / Proteínas de la Membrana / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: J Biol Chem Año: 1995 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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