Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors.
Transgenic Res
; 4(6): 369-77, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-7581517
We have used vectors derived from avian leukosis viruses to transduce exogenous genes into early somatic stem cells of chicken embryos. The ecotropic helper cell line, Isolde, was used to generate stocks of NL-B vector carrying the Neo(r) selectable marker and the Escherichia coli lacZ gene. Microinjection of the NL-B vector directly beneath unincubated chicken embryo blastoderms resulted in infection of germline stem cells. One of the 16 male birds hatched (6.25%) from the injected embryos contained vector DNA sequences in its semen. Vector sequences were transmitted to G1 progeny at a frequency of 2.7%. Neo(r) and lacZ genes were transcribed in vitro in chicken embryo fibroblast cultures from transgenic embryos of the G2 progeny.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Animales Modificados Genéticamente
/
Pollos
/
Virus de la Leucosis Aviar
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Revista:
Transgenic Res
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
1995
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Países Bajos