Synthesis of [4,5,6,8-(13)C4]guanine, a reagent for the production of internal standards of guanyl DNA adducts.

Scheller, N; Sangaiah, R; Ranasinghe, A; Amarnath, V; Gold, A; Swenberg, J A

Scheller, N; Sangaiah, R; Ranasinghe, A; Amarnath, V; Gold, A; Swenberg, J A.

Afiliación

Scheller N; Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599, USA.

Chem Res Toxicol ; 8(3): 333-7, 1995.

Article en En | MEDLINE | ID: mdl-7578918

RESUMEN

Using readily available labeled compounds, [4,5,6,8-(13)C4]guanine was synthesized in high overall yield. Intermediates as well as the final product were characterized by 1H NMR, 13C NMR, and high resolution mass spectrometry. The labeled guanine was used to generate [13C4]-labeled analogs of the guanine adducts, N2,3-ethenoguanine and 7-(2-hydroxyethyl)guanine. The application of such adducts in isotope dilution mass spectrometry was illustrated with DNA samples from rats exposed to two different mutagenic compounds, vinyl chloride and ethylene oxide.

Asunto(s)

Aductos de ADN/normas; Guanina/síntesis química; Animales; Radioisótopos de Carbono; Aductos de ADN/síntesis química; Guanina/análogos & derivados; Guanina/metabolismo; Humanos; Indicadores y Reactivos; Hígado/química; Espectroscopía de Resonancia Magnética; Ratas; Bazo/química

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aductos de ADN / Guanina Límite: Animals / Humans Idioma: En Revista: Chem Res Toxicol Asunto de la revista: TOXICOLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links