The use of filter paper monoclonal antibodies in a Dot-blot test for typing Neisseria meningitidis B.

De-Gaspari, E N; Ribeiro-Filho, A A; Zollinger, W D

De-Gaspari, E N; Ribeiro-Filho, A A; Zollinger, W D.

Afiliación

De-Gaspari EN; Secão de Immunologia, Instituto Adolfo Lutz, São Paulo, Brasil.

Braz J Med Biol Res ; 27(12): 2889-93, 1994 Dec.

Article en En | MEDLINE | ID: mdl-7550010

RESUMEN

A simple method for the collection, preservation, shipment, and testing of minute amounts of dried monoclonal antibodies for typing Neisseria meningitidis B is described. The monoclonal antibodies collected on filter paper were extracted in PBS and evaluated by Dot-blot employing whole cells of N. meningitidis B as antigen. The dried filter paper with monoclonal antibodies could be stored at room temperature for as long as 30 days without detectable changes in antibody response when used for typing outer membrane antigens of N. meningitidis B.

Asunto(s)

Anticuerpos Monoclonales; Immunoblotting/métodos; Neisseria meningitidis/clasificación; Técnicas de Tipificación Bacteriana

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Immunoblotting / Anticuerpos Monoclonales / Neisseria meningitidis Idioma: En Revista: Braz J Med Biol Res Año: 1994 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil

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