Reverse transcriptase and substrate dependence of the RNA hypermutagenesis reaction.
Nucleic Acids Res
; 23(14): 2573-8, 1995 Jul 25.
Article
en En
| MEDLINE
| ID: mdl-7544458
G-->A hypermutation is a remarkable phenomenon resulting from retroviral reverse transcription in the presence of highly biased dNTP concentrations. Of the three reverse transcriptases (RTases) available, those of human immunodeficiency virus type 1 (HIV-1), avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (MoMLV), the HIV-1 enzyme showed the greatest sensitivity to biased [dCTP]/[dTTP] ratios. The HIV-1 RTase was able to discriminate between dUTP, dITP and the four DNA precursors and was insensitive to pH. There was little preference for nucleotide contexts. A few exceptionally modified sequences were found presumably resulting from G-->A hypermutation and multiple strand transfer. This particular predilection of the HIV-1 and, by extrapolation, the lentiviral RTases towards G-->A hypermutation suggests that the phenomenon may have contributed to the remarkably elevated A content of these retroviral genomes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Mutagénesis
/
ADN Polimerasa Dirigida por ARN
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1995
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido