PCR and patch-clamp analysis of single neurons.

Sucher, N J; Deitcher, D L

Sucher, N J; Deitcher, D L.

Afiliación

Sucher NJ; Department of Neurology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Neuron ; 14(6): 1095-100, 1995 Jun.

Article en En | MEDLINE | ID: mdl-7541630

RESUMEN

The combination of patch-clamp and molecular biology techniques has made it possible to characterize the pharmacological and biophysical properties of ion channels in single neurons and to screen for expression of specific mRNAs in the same cell. Following whole-cell recording, the cytoplasm of the cell is harvested, and RNA is reverse transcribed into cDNA and amplified in PCR with primers specific for individual ion channel subunits. Additional experiments can then be designed to relate structure and function at the protein level more directly, since cells appear to regulate the composition of ion channels at least partly at the posttranscriptional stage.

Asunto(s)

Canales Iónicos/genética; Canales Iónicos/fisiología; Neuronas/fisiología; Técnicas de Placa-Clamp; Reacción en Cadena de la Polimerasa; Cartilla de ADN; ARN/aislamiento & purificación; ARN/metabolismo; ADN Polimerasa Dirigida por ARN; Receptores de N-Metil-D-Aspartato/genética; Receptores de N-Metil-D-Aspartato/fisiología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Técnicas de Placa-Clamp / Canales Iónicos / Neuronas Idioma: En Revista: Neuron Asunto de la revista: NEUROLOGIA Año: 1995 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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