Characterization of the promoter of the proto-oncogene c-kit.
Proc Natl Sci Counc Repub China B
; 19(1): 8-18, 1995 Jan.
Article
en En
| MEDLINE
| ID: mdl-7539532
In previous studies, we have characterized the nature and function of the proto-oncogene c-kit, which encodes a receptor tyrosine kinase. This receptor together with its ligand, a stem cell growth factor, constitutes a cell signaling system which is crucial for the development of hematopoietic, melanocytic and germ cells. The expression of the gene correlates with its protein functions in specific cell lineages and is temporally and spatially regulated during fetal and adult life. As a start point to study the gene regulation, we have characterized the promoter of the c-kit gene. A single transcription initiation site located 58 bases upstream of the ATG start codon has been identified. The sequence upstream to the initiation site reveals a TATA-less, non-GC rich promoter. Several potential binding sites for transcription factors pertinent to c-kit expression, such as Sp-1, GATA-1, myb and Oct-4, have been identified. Promoter activities of different lengths of the 5' sequence have been analyzed in transient expression assay. The 2.7 kb of the 5' sequence facilitates the expression of the CAT gene in several cell lines while the sequence further upstream from 2.7 to 5.0 kb shows a negative regulatory activity. This study reveals a unique promoter of the c-kit gene and provides a basis for further elucidation of the regulatory mechanism of c-kit gene expression.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proto-Oncogenes
/
Receptores del Factor Estimulante de Colonias
/
Proteínas Proto-Oncogénicas
/
Regiones Promotoras Genéticas
/
Proteínas Tirosina Quinasas Receptoras
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Proc Natl Sci Counc Repub China B
Asunto de la revista:
BIOLOGIA
Año:
1995
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China