In vitro translation of fractionated virus-specific RNA isolated from plasma of chicken infected by avian myeloblastosis virus. Unprocessed and processed myeloblastosis-associated virus env-polypeptide precursors.
Acta Virol
; 37(6): 493-501, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-7516622
The 60 S viral RNA complex isolated from leukaemic plasma of chicken infected by avian myeloblastosis virus (AMV) was denatured, the poly(A)-RNA selected and centrifuged in a linear sucrose density gradient. RNA from each fraction was translated in vitro and the products were analyzed by slab polyacrylamide gel electrophoresis (PAGE). Unprocessed primary translation product (p64env) of MAV env gene from 21 S RNA fraction was immunoprecipitated by anti-gp85 serum. If, however, this RNA was translated in the presence of dog pancreas microsomal membranes (DPM), the processed 92 K MAV glycoprotein precursor (p92env) was immunoprecipitated by anti-gp85 serum. This precursor, unlike p64env was resistant to exogenous protease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Precursores del ARN
/
Productos del Gen env
/
Procesamiento Proteico-Postraduccional
/
Virus de la Mieloblastosis Aviar
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Virol
Año:
1993
Tipo del documento:
Article
Pais de publicación:
Eslovaquia