Detection of alginate lyase by activity staining after sodium dodecil sulfate-polyacrylamide gel electrophoresis and subsequent renaturation.
Anal Biochem
; 217(1): 124-7, 1994 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-7515596
A slab gel electrophoretic method for the study of bacterial alginate lyase has been developed. By incorporating alginate in acrylamide gels, the method is based on renaturation of the enzyme after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, subsequent staining with cetylpyridinium chloride, and quantification of the spot by densitometric scanning. The molecular mass of alginate lyase can be determined from its position in the gel.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacárido Liasas
/
Proteínas Bacterianas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Biochem
Año:
1994
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos