The alpha subunit of RNA polymerase specifically inhibits expression of the porin genes ompF and ompC in vivo and in vitro in Escherichia coli.
FEMS Microbiol Lett
; 115(1): 1-6, 1994 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-7510255
Overproduction of the alpha subunit of RNA polymerase in Escherichia coli resulted in inhibition of transcription of two osmoregulated porin genes, ompF and ompC, but not of constitutively expressed housekeeping genes. Overproduction of the sigma subunit did not have any inhibitory effects. The specific inhibitory effect of the alpha subunit was also found to depend upon the OmpR protein, the transcriptional activator for ompF and ompC. These results are in general agreement with other biochemical and genetic evidence suggesting that the alpha subunit is the subunit of RNA polymerase that directly interacts with certain transcriptional activators to initiate transcription.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
ARN Polimerasas Dirigidas por ADN
/
Regulación Bacteriana de la Expresión Génica
/
Escherichia coli
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1994
Tipo del documento:
Article
Pais de publicación:
Reino Unido