Isoforms of cellular fibronectin and tenascin in amniotic fluid.
FEBS Lett
; 337(2): 167-70, 1994 Jan 10.
Article
en En
| MEDLINE
| ID: mdl-7507066
Amniotic fluid (AF) obtained from second trimester pregnancies presented extradomain (ED) A, B and an oncofetal (onc-f) domain containing isoforms of cellular fibronectin (cFn) in Western blotting of gelatin-bound polypeptides and directly of AF. Western blotting after sequential immunoprecipitation suggested at least three Fn molecules: one containing EDA and the onc-f domain and another minor component distinctly containing all the domains, and a third one only containing EDA. The immunoblotting results for EDA-cFn and onc-f-cFn were closely similar to that for total Fn, whereas in plasma samples of normal and pregnant women only traces of EDA-cFn and onc-f-cFn, but no EDB-cFn, were found. Western blotting of AF also indicated the presence of three isoforms of tenascin (Tn), M(r) 190,000 and 280,000 polypeptides earlier found in many cells, and a M(r) 200,000 polypeptide, novel for AF and not present in plasma. The results suggest a novel extracellular matrix polypeptide composition for AF.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Embarazo
/
Moléculas de Adhesión Celular Neuronal
/
Proteínas de la Matriz Extracelular
/
Fibronectinas
/
Líquido Amniótico
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
FEBS Lett
Año:
1994
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Reino Unido