Transmeiotic differentiation of male germ cells in culture.
Cell
; 75(5): 997-1006, 1993 Dec 03.
Article
en En
| MEDLINE
| ID: mdl-7504588
A cell culture system that supports the differentiation of male germ cells through meiosis is described. It takes advantage of the properties of a cell line, 15P-1, established from testicular cells of transgenic mice that express the large T protein of polyoma virus in the seminiferous epithelium. This line exhibits features characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Cells of the 15P-1 type support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generated tetrads of haploid cells with the morphology of round spermatids and initiated protamine transcription.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células de Sertoli
/
Espermatogénesis
/
Meiosis
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos