A human monoclonal antibody, produced following in vitro immunization, recognizing an epitope shared by HLA-A2 subtypes and HLA-A28.
Tissue Antigens
; 42(1): 27-34, 1993 Jul.
Article
en En
| MEDLINE
| ID: mdl-7504327
In vitro immunization and subsequent immortalization of peripheral blood cells of a multiparous woman has resulted in the production of a stable human mouse heterohybridoma, 5C2A2, secreting an HLA-A2/A28-specific human monoclonal antibody. Although possibly exposed to HLA-A2 by transfusions, the cell donor showed no HLA-A2-specific serum antibodies. The present protocol for in vitro immunization includes the elimination of suppressor cells from the responder cell population, the presence of irradiated allogeneic lymphocytes as a source of antigen, as well as stimuli--recombinant interleukin-2 and a B-cell specific nucleoside analogue--causing the proliferation of B lymphocytes, prior to immortalization. The ability of the antibody 5C2A2 to detect all known HLA-A2 subtypes, except A2.3, and A28, allows identification of the serological epitope on the HLA-A2 molecule. Application of this in vitro immunization method allows the production of a set of HLA monoclonal antibody-secreting human hybridomas, independent of the existence of serum HLA antibodies in the lymphocyte donors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos HLA-A
/
Antígeno HLA-A2
/
Inmunización
/
Anticuerpos Monoclonales
/
Epítopos
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Tissue Antigens
Año:
1993
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido