dpa, a member of the MCM family, is required for mitotic DNA replication but not endoreplication in Drosophila.
EMBO J
; 14(21): 5387-98, 1995 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-7489728
We have isolated the Drosophila disc proliferation abnormal (dpa) gene, a member of the MCM family of DNA replication factors. Members of this family of proteins are required for DNA replication in yeast. A dpa null mutant dies during pupal stages because imaginal tissues necessary for the formation of the adult fly fail to proliferate normally. Beginning in late embryogenesis BrdU labeling reveals DNA replication defects in mitotically proliferating cells. In contrast, dpa is dispensable for endoreplication, a specialized cell cycle consisting of consecutive rounds of S phases without intervening mitosis. Our studies suggest an essential role for dpa in mitotic DNA replication but not in endoreplication. Thus, dpa is not a general replication factor but may play a specialized regulatory role in DNA replication.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Fúngicas
/
Proteínas de Ciclo Celular
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Proteínas de Drosophila
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Proteínas de Unión al ADN
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Replicación del ADN
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Drosophila
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido