[Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis]. / Opredelenie somatotropina i prolaktina dodetsilsul'fat-élektroforezom v poliakrilamidnom gele.
Vopr Med Khim
; 26(1): 71-5, 1980.
Article
en Ru
| MEDLINE
| ID: mdl-7368631
Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Prolactina
/
Dodecil Sulfato de Sodio
/
Hormona del Crecimiento
/
Electroforesis en Gel de Poliacrilamida
Límite:
Animals
Idioma:
Ru
Revista:
Vopr Med Khim
Año:
1980
Tipo del documento:
Article
Pais de publicación:
Rusia