Specific interaction of corticosteroids with binding sites in the plasma membranes of the rat anterior pituitary gland.
J Endocrinol
; 79(2): 215-22, 1978 Nov.
Article
en En
| MEDLINE
| ID: mdl-731146
The binding of glucocorticoids to a crude fraction of rat pituitary plasma membranes and to solubilized membrane proteins was measured. The binding characteristics were similar to those exhibited by transcortin: radioactive corticosterone was bound to a greater extent than radioactive dexamethasone and labelled corticosterone, but not labelled dexamethasone, was displaced by unlabelled corticosterone, deoxycorticosterone and progesterone. A Scatchard plot of the binding data revealed the presence of a binding material with a dissociation constant of about 3.2 nmol/l, which sedimented at 4S after sucrose density-gradient centrifugation. It was found that the number of binding sites was inversely related to the concentration of corticosterone in the circulation and was increased after long-term adrenalectomy. These data suggest that a material similar to transcortin is complexed to the plasma membrane of rat pituitary cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenohipófisis
/
Corticosterona
/
Receptores de Glucocorticoides
/
Receptores de Esteroides
Límite:
Animals
Idioma:
En
Revista:
J Endocrinol
Año:
1978
Tipo del documento:
Article
Pais de publicación:
Reino Unido