[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation]. / Wplyw czynników antykoagulacyjnych na izolacje limfocytów technika wirowania w gradiencie gestosci.
Acta Haematol Pol
; 12(2): 77-83, 1981.
Article
en Pl
| MEDLINE
| ID: mdl-7304089
The authors compared the efficiency of isolation and the purity of lymphocytes obtained by the method of centrifugation in a gradient of ficoll and uropoline from blood obtained on ACD fluid and on heparin. Isolation of lymphocytes from the blood obtained on heparin was more efficient (60%) and useful. For removal of platelets it was necessary to use additional centrifugation in a saccharose gradient or application of aggregating agents during isolation of lymphocytes obtained on heparin or ACD fluid.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Heparina
/
Linfocitos
/
Separación Celular
/
Centrifugación por Gradiente de Densidad
/
Citratos
Límite:
Humans
Idioma:
Pl
Revista:
Acta Haematol Pol
Año:
1981
Tipo del documento:
Article
Pais de publicación:
Polonia