[Alanine transport in a mammalian cell culture]. / Transport alanina v kul'ture kletok mlekopitaiushchikh.
Tsitologiia
; 24(6): 667-72, 1982 Jun.
Article
en Ru
| MEDLINE
| ID: mdl-7123655
The transport of L-alanine in cultured 3T3, 3T6 and CHO cells has been studied. The estimated Michaelis kinetic constants KM and Vmax for 3T3, 3T6 and CHO cells were 0.6 mM and 3 mM/min, 2.5 mM and 8.3. mM/min, 0.7 mM and 8.4 mM/min, respectively. Alanine transport was inhibited by glycine, serine, alpha-aminoisobutyric acid, methionine, and in a lesser degree by leucine and valine. Glycine depressed alanine transport with the constant of inhibition (Ki) similar to the constant of glycine transport (Km). Alanine transport was strictly diminished in a Na+-dependent medium at the expense of the increase of Km for transport. It is concluded that in 3T3, 3T6 and CHO cells, alanine is transported by the Na+-dependent "A" system.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alanina
/
Fibroblastos
Límite:
Animals
Idioma:
Ru
Revista:
Tsitologiia
Año:
1982
Tipo del documento:
Article
Pais de publicación:
Rusia