Screening for deficiencies in the classical and alternative pathways of complement by hemolysis in gel.
Acta Pathol Microbiol Scand C
; 89(3): 161-6, 1981 Jun.
Article
en En
| MEDLINE
| ID: mdl-7032205
Two assays based on hemolysis in gel were assessed for screening complement (C) component deficiencies. In one assay sensitized sheep erythrocyte (EA) were incorporated in agarose gel containing Ca2+ and Mg2+, in the other guinea pig erythrocytes (GpE) were used in the presence of Mg2+and EGTA. With few exceptions, fresh samples from healthy individuals produced homogeneous areas of complete hemolysis in both assays. Clearly aberrant patterns were observed in approximately 4% of healthy blood donors. Sera from patients having complete deficiencies of Clq, C2 or C4 produced clear lysis of GpE only, whereas in sera lacking C3 or C8 lysis was grossly impaired in both assays. Properdin deficient serum produced very slight lysis of GpE but normal lysis of EA. Reconstitution of these C-deficient sera gave normal lysis. Together, the two assays supplement immunochemical C3 and C4 determinations for screening out C disorders.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Activación de Complemento
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Pathol Microbiol Scand C
Año:
1981
Tipo del documento:
Article
Pais de publicación:
Dinamarca