The in vitro inhibitory effect on thrombin by 2,3-diphosphoglycerate.

Del Principe, D; Strappini, P M; Del Vecchio, S; Menichelli, A; Biancini, G; Cosmi, E V; Bastianon, V; D'Arcangelo, C

Del Principe, D; Strappini, P M; Del Vecchio, S; Menichelli, A; Biancini, G; Cosmi, E V; Bastianon, V; D'Arcangelo, C.

Thromb Haemost ; 46(3): 581-3, 1981 Oct.

Article en En | MEDLINE | ID: mdl-7031980

RESUMEN

Thrombin incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, alpha-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, alpha-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.

Asunto(s)

Ácidos Difosfoglicéricos/farmacología; Trombina/antagonistas & inhibidores; Animales; Anticoagulantes; Coagulación Sanguínea/efectos de los fármacos; Bovinos; Cromatografía por Intercambio Iónico/métodos; Depresión Química; Fibrina/fisiología; Fibrinógeno/fisiología; Fibrinopéptido A/antagonistas & inhibidores; Fibrinopéptido B/antagonistas & inhibidores; Humanos; Técnicas In Vitro; Péptido Hidrolasas/sangre

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombina / Ácidos Difosfoglicéricos Límite: Animals / Humans Idioma: En Revista: Thromb Haemost Año: 1981 Tipo del documento: Article Pais de publicación: Alemania

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