Effect of snap freezing, dithiothreitol and storage on estimations of estrogen receptor sites.
Clin Chim Acta
; 88(2): 337-43, 1978 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-699329
Estrogen receptor assays were performed on human myometrium in order to determine optimal conditions for certain steps of the assay. The findings indicate that: (1) tissue should be snap-frozen in liquid nitrogen prior to assay, (2) dithiothreitol does not increase measurable binding site numbers, (3) estrogen receptors in the cytosol fraction are unstable and should be stored in excess of 1 day; (4) if storage prior to receptor assay is unavoidable, tissue should be stored at -20 degrees C or -70 degrees C for no longer than 1 week.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores de Estrógenos
Límite:
Female
/
Humans
Idioma:
En
Revista:
Clin Chim Acta
Año:
1978
Tipo del documento:
Article
Pais de publicación:
Países Bajos