Uptake of iopanoic acid and its glucuronide conjugate by rat hepatocytes in primary culture.
Proc Soc Exp Biol Med
; 173(4): 506-11, 1983 Sep.
Article
en En
| MEDLINE
| ID: mdl-6889314
Uptake of iopanoic acid (IOP) and iopanoate glucuronide (IOP-G) was studied in 3-day primary cultures of rat hepatocytes isolated by the collagenase perfusion method. 125I activity of cells after incubation with 125I-IOP (10-100 microM) and 125I-IOP-G (10-100 microM) was used as a measure of uptake. At each concentration, uptake was linear for the first 45 sec. In the absence of albumin, the initial uptake velocity was directly proportional to the concentration or IOP or IOP-G and was nonsaturable up to 100 microM. The calculated uptake rate constants for IOP and for IOP-G were 0.059 and 0.048 nmole/(mg protein X min X microM), respectively. IOP uptake was not inhibited by sodium taurocholate nor by the contrast agents iodipamide, ipodate, and iopronic acid. The data indicate that the enhancement of IOP excretion by bile salts noted in vivo does not occur at the uptake step and that the hepatic uptake of both IOP and IOP-G in the absence of albumin is limited by diffusion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Yopanoico
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
Proc Soc Exp Biol Med
Año:
1983
Tipo del documento:
Article
Pais de publicación:
Estados Unidos