Tetanus toxin-[2,3-3H]propionamid. Synthesis, specific activity, immunoreactivity, toxicity and ascent into the spinal cord.
Arch Toxicol
; 52(4): 303-10, 1983 Apr.
Article
en En
| MEDLINE
| ID: mdl-6870543
Tetanus toxin was tritiated with various amounts of N-succinimidyl-[2,3-3H]propionate. A tenfold molar excess of reagent over toxin was used for the introduction of about 5 nmol of [2,3-3H]propionate into 1 nmol of toxin, corresponding to a specific activity of about 48 MBq/mg protein. The labelling procedure did not change significantly the immunoreactivity of tetanus toxin, but reduced the toxicity in mice from 12 ng/kg to 16 ng/kg. Tetanus toxin-[2,3-3H]propionamid was carried through the peripheral nerves into the spinal cord. It could be used instead of 125I-labelled toxin for the detection of anti-tetanus toxin antibodies with a detection limit of 0.25 mU/ml of serum.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Propionatos
/
Médula Espinal
/
Toxina Tetánica
Límite:
Animals
Idioma:
En
Revista:
Arch Toxicol
Año:
1983
Tipo del documento:
Article
Pais de publicación:
Alemania