[Preparation of protoplast-like structures of Pseudomonas putida and their reversion to bacterial forms]. / Poluchenie protoplastopodobnykh struktur Pseudomonas putida i ikh reversiiia v bakterial'nye formy.
Mikrobiologiia
; 51(4): 628-31, 1982.
Article
en Ru
| MEDLINE
| ID: mdl-6755196
A technique is proposed for the preparation of Pseudomonas putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing mono-and divalent cations. Pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 M) for 90 min at the pH 7.9 to 8.0 is a prerequisite for the formation of 'protoplasts'. Under these conditions, the yield of 'protoplasts' reached 99.9% of the total cell number. The protoplast-like structures are capable of reversing into the original bacterial forms in a minimal hypertonic medium containing amino acids which are components of the cell wall. The level of reversion reaches 10% for certain strains.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Protoplastos
/
Pseudomonas
Idioma:
Ru
Revista:
Mikrobiologiia
Año:
1982
Tipo del documento:
Article
Pais de publicación:
Rusia