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Simple assay of estrogen in urine during pregnancy by means of latex agglutination inhibition reaction.
Biol Res Pregnancy Perinatol ; 5(2): 74-7, 1984.
Article en En | MEDLINE | ID: mdl-6743735
A simple and rapid method, latex agglutination inhibition reaction (LAIR), using anti-estriol-16-glucuronide coupled with BSA for estimating estrogen in urine has been developed. Agglutination of the latex reagent by the antiserum occurs within 3 min. The agglutination, however, was completely inhibited by 100 ng/ml or more of estriol. There was a good correlation in results obtained by LAIR and by colorimetry, RIA and GLC. Urine samples (3329) obtained at various gestational weeks were assayed. The samples from 94% of the normal pregnant patients in the third trimester revealed 10 micrograms/ml or more of estrogens. Although the assay is semiquantitative, it may be useful in screening for fetal well-being in high-risk pregnancies.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Embarazo / Estrógenos Límite: Female / Humans Idioma: En Revista: Biol Res Pregnancy Perinatol Año: 1984 Tipo del documento: Article Pais de publicación: Alemania
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Embarazo / Estrógenos Límite: Female / Humans Idioma: En Revista: Biol Res Pregnancy Perinatol Año: 1984 Tipo del documento: Article Pais de publicación: Alemania