Identification of the plasminogen activator(s) produced by the transformed liver cell line, SK-HEP-1.
Thromb Res
; 32(1): 29-34, 1983 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-6686346
The availability of a cell line derived from an adenocarcinoma of the liver has made it possible to study the plasminogen activator(s) (PA) biosynthesized in culture by liver cells. Conditioned cultured media purified on fibrin-celite, benzamidine-Sepharose and immunoabsorbent anti-urokinase columns have shown the presence of multiple plasminogen activators when separated on sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE). These PAs differ in molecular weight but all are urokinase-like based on their reaction with goat anti-urokinase and rabbit anti-tissue activator. Subcellular fractionation of the cultured cells shows the presence of activator in both the cytoplasmic and membrane fractions, but the higher molecular weight forms appear primarily in the cytoplasm.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Activadores Plasminogénicos
/
Hígado
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Thromb Res
Año:
1983
Tipo del documento:
Article
Pais de publicación:
Estados Unidos